Coding

Part:BBa_K3190301:Design

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-14)


TPST1: Tyrosylprotein sulfotransferase 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1057
    Illegal XbaI site found at 511
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1057
    Illegal NheI site found at 960
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1057
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1057
    Illegal XbaI site found at 511
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1057
    Illegal XbaI site found at 511
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TPST 1 and 2 are the enzymes, that facilitate tyrosine sulfation, a post translational modification essential for the function of the human LHCGR receptor. Yeast do not have these necessary enzymes (TPST1 and TPST2) by itself, which is why we use it in our construct.



Source

Homo sapiens

References